Journal: International Journal of Molecular Sciences

Article Title: Pro-Inflammatory Macrophage Phenotype Skewing Induced by Tumor Treating Fields (TTFields)

doi: 10.3390/ijms262412086

Figure Lengend Snippet: TTFields activate GEF-H1 and drive macrophage transcriptional reprogramming via c-Jun and p65. BMDMs were polarized to either an M1 state (IFN-γ + LPS) or an M2 state (IL-4) and then exposed to TTFields. ( A , B ) Western blot analysis of phosphorylated GEF-H1 (Ser886) following TTFields treatment at 15, 30, and 60 min in M1 and M2 macrophages. ( A , C ) Phosphorylation of NF-κB p65 (Ser536) in BMDMs treated with TTFields. ( A , D ) Phosphorylation of c-Jun (Ser63) in TTFields-treated macrophages. Densitometric analysis for phosphorylation fold change is shown as mean ± SEM; N ≥ 3. * p < 0.05, and ** p < 0.01; one-way ANOVA followed by Dunnett test compared with time zero.

Article Snippet: phosphorylated GEF-H1 (Ser886) , 1:1000 , 14143 , Cell signaling *.

Techniques: Western Blot, Phospho-proteomics

Journal: International Journal of Molecular Sciences

Article Title: Pro-Inflammatory Macrophage Phenotype Skewing Induced by Tumor Treating Fields (TTFields)

doi: 10.3390/ijms262412086

Figure Lengend Snippet: TTFields activate GEF-H1 and drive macrophage transcriptional reprogramming via c-Jun and p65. BMDMs were polarized to either an M1 state (IFN-γ + LPS) or an M2 state (IL-4) and then exposed to TTFields. ( A , B ) Western blot analysis of phosphorylated GEF-H1 (Ser886) following TTFields treatment at 15, 30, and 60 min in M1 and M2 macrophages. ( A , C ) Phosphorylation of NF-κB p65 (Ser536) in BMDMs treated with TTFields. ( A , D ) Phosphorylation of c-Jun (Ser63) in TTFields-treated macrophages. Densitometric analysis for phosphorylation fold change is shown as mean ± SEM; N ≥ 3. * p < 0.05, and ** p < 0.01; one-way ANOVA followed by Dunnett test compared with time zero.

Article Snippet: GEF-H1 , 1:1000 , 4076 , Cell signaling *.

Techniques: Western Blot, Phospho-proteomics

Journal: International Journal of Molecular Sciences

Article Title: Pro-Inflammatory Macrophage Phenotype Skewing Induced by Tumor Treating Fields (TTFields)

doi: 10.3390/ijms262412086

Figure Lengend Snippet: TTFields-induced iNOS expression and cytokine secretion in polarized macrophages. BMDMs were polarized with IFN-γ or IL-4 and then exposed to TTFields with or without pharmacological in-hibitors, or LPS. iNOS protein expression was assessed by flow cytometry. ( A , B ) Comparison of iNOS levels in IFN-γ–primed macrophages treated with TTFields or LPS, and the effect of MyD88 inhibition (TJ-M2010-5) or ROCK inhibition (Y-27632). ( C – E ) Western blot analysis of GEF-H1 and NF-κB p65 activation in IFN-γ–primed macrophages exposed to TTFields. ( F ) Cytokine and chemokine secretion profile measured by multiplex analysis in supernatants from IFN-γ–primed BMDMs. mean ± SEM; N = 3. * p < 0.05, ** p < 0.01, and *** p < 0.001; Student’s t -test.

Article Snippet: GEF-H1 , 1:1000 , 4076 , Cell signaling *.

Techniques: Expressing, Flow Cytometry, Comparison, Inhibition, Western Blot, Activation Assay, Multiplex Assay